EDUCATION

2009, Ph.D., Biomedical Engineering
    Advisor: Dr. Marc Madou
    University of California, Irvine

2006, M.S., Biomedical Engineering
    University of California, Irvine

2005, B.S., Electrical Engineering w/Biomedical Engineering Option
    University of California, Los Angeles

Skills Overview

Manufacturing: Polymer CNC machining (micromachining), bonding, surface treatments, photolithography, cleanrooms

Bio/Chemical: DNA/RNA assays (amplification reactions), immunoassays, surface immobilization, microscopy

Instrumentation: Electrical, optical (fiber-optics, LEDs, PMTs), thermal (RTDs, TEs, TCs), magnetic

Software: CAD (SolidWorks, Pro/E, AutoCAD), CAM (Pro/E), LabVIEW, Windows, Mac, Linux

Publications: 25+ conferences and papers including 1st authorships in industry-leading publications (IVD Tech.)

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Project EXPERIENCE Highlights

LEAD Engineer & RESEARCHEr for Microfluidic Immunoassay SYSTEM with EMD Millipore: International collaboration with Millipore to develop a microfluidic system capable of performing automated immunoassays (FLISA format)

Key Outputs: created the reagent delivery device and integrated it with the assay, surface chemistry, and optical instrument

Engineer & RESEARCHEr for Microfluidic Infectious Disease Diagnostics: Multi-million dollar international collaboration to develop a microfluidic system for nucleic acid-based respiratory virus diagnostics (PCR + microarray format)

Key Outputs: created microfluidic PCR and sample prep. (lysis) devices and integrated them into a single fluidic system

LEAD Engineer & Co-Founder of iGlyko, Inc for glucose sensing: Worked in the business, financial, and technical arms of the company for the development of a fluorescence-based continuous glucose-sensing intravenous catheter

Key Outputs: optimized hydrogel chemistries, fabricated fiber-optic systems, and established proof-of-concept in whole blood

Nucleic Acid ASSAY R&D Biochemist for Gen-Probe, Inc: Developed and optimized modified versions of real-time transcription-meditated amplification (TMA) assays as a member of Gen-Probe’s biochemistry R&D department

Key Outputs: established proof-of-concept and then optimized an alternate assay format for prostate cancer detection

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ACADEMIA EXPERIENCE

2008-current : PEER Reviewer of Journal Publications

1. Perform peer reviews of manuscripts submitted to various scientific journals, including IEEE Journal of MEMS, Sensors and Actuators, Microfluidics and Nanofluidics, Lab on a Chip, and various others
   

2007-2008 : Student Representative on UC Irvine, Biomedical Engineering Graduate
Admissions Committee

1. Served with BME faculty to help review applications and decide on admission of graduate students
   

2. Tasks included reading and sorting applications, researching unknown schools, initiating student contact, and helping make final admit decisions
   

JOURNAL pUBLICATIONS

1. 2010 : Sensors and Actuators B: Chemical Journal Paper

1. J. Siegrist, T. Kazarian, C. Ensor, S. Joel, M. Madou, P. Wang, and S. Daunert, 2010, Continuous Glucose Sensor Using Novel Genetically-Engineered Binding Polypeptides Towards In vivo Applications, Sensors and Actuators B: Chemical, 149, 51-58. Link to PDF
   

2. 2010 : Lab on a Chip Review paper

1. R. Gorkin, J. Park, J. Siegrist, M. Amasia, B. Lee, J. Park, J. Kim, H. Kim, M. Madou, and Y. Cho, 2010, Centrifugal Microfluidics for Biomedical Applications, Lab on a Chip, 10, 1758–1773. Link to PDF
   

3. 2010 : Lab on a Chip journal paper   

1. J. Siegrist, M. Amasia, N. Singh, D. Banerjee, and M. Madou, 2010, Numerical Modeling and Experimental Validation of Uniform Microchamber Filling in Centrifugal Microfluidics, Lab on a Chip, 10, 876-886. Link to PDF
   

4. 2010 : Lab on a ChiP journal paper   

1. J. Siegrist, R. Gorkin, M. Bastien, G. Stewart, R. Peytavi, H. Kido, M. Bergeron, and M. Madou, 2010, Validation of a Centrifugal Microfluidic Sample Lysis and Homogenization Platform for Nucleic Acid Extraction with Clinical Samples, Lab on a Chip, 10, 363-371. Link to PDF
   

5. 2009 : Microfluidics and Nanofluidics journal paper

1. J. Siegrist, R. Gorkin, L. Clime, E. Roy, R. Peytavi, H. Kido, M. Bergeron, T. Veres, and M. Madou, 2010, Serial Siphon Valving for Centrifugal Microfluidic Platforms, Microfluidics and Nanofluidics, 9, 55-63. Link to PDF
   

6. 2007 : Colloids and Surfaces B Journal Paper

1. G. Jia, J. Siegrist, C. Deng, J. Zoval, G. Stewart, R. Peytavi, A. Huletsky, M. Bergeron, and M. Madou, 2007, A Low-Cost, Disposable Card for Rapid Polymerase Chain Reaction, Colloids and Surfaces B: Biointerfaces Special Edition, 58, 52-60. Link to PDF
   

Select CONFERENCES

1. 2011 : IEEE MEMS Conference Abstract & Poster
Cancun, Mexico

1. J. Siegrist, L. Zavattoni, and J. Ducrée, 2011, Centrifugo-Magnetophoretic Separation and Routing of Particles, Proceedings of IEEE MEMS 2011.
   

2. 2010 : microTAS Conference Abstract & Poster
Groningen, The Netherlands

1. M. Amasia, J. Siegrist, and M. Madou, 2010, Large-Volume Centrifugal Microfluidic Device for Whole Blood Sample Preparation, Proceedings of MicroTAS 2010.
   

3. 2010 : American Diabetes Association Conference Abstract & Poster
Orlando, FL                                                                                                                                                                                      

1. D. Tobler, J. Siegrist, T. Kazarian, M. Madou, P. Wang, and S. Daunert, 2010, A Novel Genetically Engineered Biosensor for Real-Time In vivo Glucose Sensing, Proceedings of American Diabetes Association’s 70th Scientific Sessions 2010.
   

4. 2010 : Lab-on-a-Chip European Congress Abstract & Poster
Dublin, Ireland   

1. R. Burger, I. Dimov, J. Siegrist, and J. Ducrée, 2010, Capture and Isolation of Cells Using Geometrical Barriers on a Sedimentation-Based Centrifugal Microfluidic Platform, Proceedings of Lab-on-a-Chip European Congress 2010.
   

5. 2009 : Oak Ridge Conference: Frontiers in Clinical Diagnostics Abstract & Poster
Baltimore, MD

1. N. Nelson, D. Lyakhov, S. Phelps, J. Chelliserrykattil, J. Carlson, M. Kaminsky, P. Gordon, S. Hashima, T. Ngo, J. Siegrist, and S. Brentano, 2009, Quantitative Detection of PCA3, PSA, and Internal Control in a Quantitative, Multiplex, Universal Real-time Transcription-Meditated Amplification Assay Format, Proceedings of Oak Ridge Conference: Frontiers in Clinical Diagnostics 2009.
   

6. 2008 : microTAS Conference Abstract & Poster
San diego, CA

1. E. Roy, J. Siegrist, R. Peytavi, G. Diaz-Quijada, H. Roberge, F. Normandin, G. Jia, J. Zoval, M. Madou, M. Bergeron, M. Dumoulin, and T. Veres, 2008, Thermoplastic Elastomer (TPE) Block Copolymers, A New Material Platform for Microfluidics: Proof-of-concept for Complex Siphon Valving on a CD, Proceedings of MicroTAS 2008.
   

7. 2008 : Frontiers in Biomedical Devices Conference Abstract & Presentation
irvine, CA

1. J. Siegrist, M. Amasia, H. Kido, J. Zoval, and M. Madou, 2008, Microfluidic CD-Based Systems Towards Rapid Anthrax Detection in Whole Blood, Proceedings of Frontiers in Biomedical Devices 2008.
   

8. 2007 : Society for Experimental Mechanics Conference Paper & Presentation
springfield, mA

1. J. Siegrist, G. Jia, H. Kido, J. Zoval, G. Stewart, D. Gagné, R. Peytavi, A. Huletsky, M. Bergeron,
   and M. Madou, 2007, Centrifugal (CD) Microfluidic Platforms for Nucleic Acid Analysis, Proceedings of SEM 2007.
   

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BOOK CHAPTERS

1. 2009 : Fundamentals of Microfabrication & Nanotechnology, 3rd edition   

1. Select text, figure, and review/editing contributions to Chapters 5 and 7 of Fundamentals of Microfabrication and Nanotechnology, 3rd Edition, M. Madou, 2009, CRC Press: Boca Raton, London, New York, and Washington D.C.
   

Select PATENTS AND DISCLOSURES

1. 2010 : US Provisional Patent   

1. D. Tobler and J. Siegrist, 2010, Catheter Based Sensing System.
   

2. 2010 : US Patent APPLICATION   

1. J. Siegrist, R. Gorkin, R. Peytavi, M. Madou, H. Kido, M. Amasia, E. Roy, and T. Veres, 2009, Centrifugal Microfluidic System for Nucleic Acid Sample Preparation, Amplification, and Detection - US 61/252,917.
   

3. 2009 : Software Copyright   

1. J. Siegrist, 2009, Multi-Level Gain-Scheduled PID Control Algorithm for Rapid & Stable Thermocycling using Thermoelectric Devices.
   

4. 2009 : International Patent Application   

1. J. Siegrist, M. Madou, P. Wang, S. Daunert, E. Moschou, S. Joel, K. Turner, L. Bachas, B. Haley, and L. Rowe, 2009, Device for Detection of Molecules of Interest - WO 2009/021039.
   

Other Select publications, press, And Presentations

1. 2010 : IVD Technology Webcast   

1. J. Siegrist, Microfluidic Applications for IVDs, IVD Technology Webcast, Nov. 16, 2010. Link to Online Info
   

2. 2010 : IVD Technology DX Directions Article   

1. J. Siegrist, R. Peytavi, M. Bergeron, and M. Madou, 2010, Microfluidics for IVD Analysis: Triumphs and Hurdles of Centrifugal Platforms - Part 3: A Vision for Tomorrow, IVD Technology DX Directions, (March). Link to Online Article    Link to PDF
   

3. 2010 : Chips & TIPS   

1. J. Siegrist, M. Amasia, and M. Madou, 2010, Fast-iteration Prototyping and Bonding of Complex Plastic Microfluidic Devices,
   Chips & Tips, online. Link to Online Tip
   

4. 2010 : IVD Technology Magazine Article   

1. J. Siegrist, R. Peytavi, M. Bergeron, and M. Madou, 2010, Microfluidics for IVD Analysis: Triumphs and Hurdles of Centrifugal Platforms - Part 2: Centrifugal Microfluidics, IVD Technology, 16 (January/February). Link to Online Article
   

5. 2009 : Doctoral Dissertation

1. J. Siegrist, 2009, Centrifugal Microfluidic Platforms Towards Sample-to-Answer Nucleic Acid Diagnostics. Link to Dissertation Online
   

6. 2009 : IVD Technology Magazine Article (Cover Article)   

1. J. Siegrist, R. Peytavi, M. Bergeron, and M. Madou, 2009, Microfluidics for IVD Analysis: Triumphs and Hurdles of Centrifugal Platforms - Part 1: Molecular Fundamentals, IVD Technology, 15 (November/December). Link to Online Article
   

7. 2007 : newspaper article   

1. DNA tests get faster, smaller, cheaper, Orange County Register, Print & Online (June 28th, 2007). Link to Online Article
   

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awards

1. UC Irvine Graduate Dean’s Dissertation Fellowship : 2009
   

2. UC Graduate Student Researcher Travel Award : 2007
   

3. UC Irvine Biomedical Engineering Graduate Transition Fellowship : 2005
   

other

1. Academy Leadership - Leadership Bootcamp : 2010
   

    -Intensive, hands-on, 3-day leadership seminar led by previous members of the US Military

    -Topics focused on included communication, motivation, conflict management, coaching, and goal setting

1. OCTANe Member : 2007-2010
   

2. BiON Cleanroom Facility Set Up : 2007-2008
   

    -Worked as part of a team to establish and set up a biomedical-oriented cleanroom facility at UC Irvine

1. BMES Member : 2005-Current   
   

2. IEEE Member : 2002-Current
   

3. Peet’s Coffee & Tea : 1998-2005
   -Worked on and off through high school and college as a barista, sales person, and financial manager
   

References

1. Available Upon Request
   

objective

Obtain permanent employment as a biomedical research and development engineer in the field of in vitro diagnostics
I want to contribute to revolutionary products that will drastically improve healthcare. My career goal is to manage industry projects for devices focused on the diagnostics market (clinical and/or point-of-care).

Technical pROFILE

1. Strong interdisciplinary engineering and biomedical background
   

2. Extensive experience with technology and systems integration
   

3. Design, prototyping, and manufacture of polymer meso/microfluidic devices
   

4. Hardware and software development for instrumentation support
   

5. Assay development including surface chemistries and signal transduction
   

Personal pROFILE

1. Self-motivated and hard working
   

2. Persistent and creative problem solver
   

3. Excels in challenging environments
   

4. Thrives in team or individual settings
   

5. Enjoys branching out to learn new topics and gain additional skills
   

6. Natural leader with excellent communication and leadership skills
   

7. Strong entrepreneurial drive
   

8. Exceptional writing skills
   

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